ChloroquineV1, Main, Exploration, bibRecord, 001A57

Functional expression of TLR9 is associated to the metastatic potential of human lung cancer cell: functional active role of TLR9 on tumor metastasis.

Identifieur interne : 001A57 ( Main/Exploration ); précédent : 001A56; suivant : 001A58

Functional expression of TLR9 is associated to the metastatic potential of human lung cancer cell: functional active role of TLR9 on tumor metastasis.

Auteurs : Tao Ren [République populaire de Chine] ; Zhen-Ke Wen ; Zhong-Min Liu ; Yong-Jie Liang ; Zhong-Liang Guo ; Lin Xu

Source :

Cancer biology & therapy [ 1555-8576 ] ; 2007.

RBID : pubmed:17986857

Descripteurs français

KwdFr :
- Cytométrie en flux, Humains, Interleukine-1 alpha (biosynthèse), Interleukine-8 (biosynthèse), Matrix metalloproteinase 2 (analyse), Métastase tumorale, Oligodésoxyribonucléotides (pharmacologie), Récepteur-9 de type Toll-like (analyse), Récepteur-9 de type Toll-like (physiologie), Technique de Western, Transduction du signal, Tumeurs du poumon (anatomopathologie).
MESH :
- analyse : Matrix metalloproteinase 2, Récepteur-9 de type Toll-like.
- anatomopathologie : Tumeurs du poumon.
- biosynthèse : Interleukine-1 alpha, Interleukine-8.
- pharmacologie : Oligodésoxyribonucléotides.
- physiologie : Récepteur-9 de type Toll-like.
- Cytométrie en flux, Humains, Métastase tumorale, Technique de Western, Transduction du signal.

English descriptors

KwdEn :
- Blotting, Western, Flow Cytometry, Humans, Interleukin-1alpha (biosynthesis), Interleukin-8 (biosynthesis), Lung Neoplasms (pathology), Matrix Metalloproteinase 2 (analysis), Neoplasm Metastasis, Oligodeoxyribonucleotides (pharmacology), Signal Transduction, Toll-Like Receptor 9 (analysis), Toll-Like Receptor 9 (physiology).
MESH :
- chemical , analysis : Matrix Metalloproteinase 2, Toll-Like Receptor 9.
- chemical , biosynthesis : Interleukin-1alpha, Interleukin-8.
- pathology : Lung Neoplasms.
- chemical , pharmacology : Oligodeoxyribonucleotides.
- chemical , physiology : Toll-Like Receptor 9.
- Blotting, Western, Flow Cytometry, Humans, Neoplasm Metastasis, Signal Transduction.

Abstract

CpG-oligonucleotides (CpG-ODN), which induce signaling through Toll-like receptor 9 (TLR9), are widely used as adjuvants in therapy against cancer. However, tumor cells express functional TLR9 were recently reported and the immune effect of CpG ODN on tumor cells remains unclear. Here we investigated the direct effects of CpG ODN on human tumor cell line 95D cells using flow cytometric analysis and Western blotting. We found strongly high expression of TLR9 in 95D cells. Stimulation of 95D cells with CpG ODN induced significantly elevated secretion of IL-1alpha and IL-8, as well as the expression of CXCR4, ICAM-1 and MMP-2. Furthermore, the invasion of 95D cells and TLR9 modifying 95C cells were significantly enhanced by stimulation of CpG ODN, which could be abrogated by inhibitory CpG ODN and chloroquine. These results suggest that functionally active TLR9 is expressed on human tumor cell lines, and may represent a novel insight on the role of TIL9 agonist used in tumor immunotherapy.

DOI: 10.4161/cbt.6.11.4826
PubMed: 17986857

Affiliations:

République populaire de Chine

Le document en format XML

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<front><div type="abstract" xml:lang="en">CpG-oligonucleotides (CpG-ODN), which induce signaling through Toll-like receptor 9 (TLR9), are widely used as adjuvants in therapy against cancer. However, tumor cells express functional TLR9 were recently reported and the immune effect of CpG ODN on tumor cells remains unclear. Here we investigated the direct effects of CpG ODN on human tumor cell line 95D cells using flow cytometric analysis and Western blotting. We found strongly high expression of TLR9 in 95D cells. Stimulation of 95D cells with CpG ODN induced significantly elevated secretion of IL-1alpha and IL-8, as well as the expression of CXCR4, ICAM-1 and MMP-2. Furthermore, the invasion of 95D cells and TLR9 modifying 95C cells were significantly enhanced by stimulation of CpG ODN, which could be abrogated by inhibitory CpG ODN and chloroquine. These results suggest that functionally active TLR9 is expressed on human tumor cell lines, and may represent a novel insight on the role of TIL9 agonist used in tumor immunotherapy.</div>
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Serveur d'exploration Chloroquine

Functional expression of TLR9 is associated to the metastatic potential of human lung cancer cell: functional active role of TLR9 on tumor metastasis.

Functional expression of TLR9 is associated to the metastatic potential of human lung cancer cell: functional active role of TLR9 on tumor metastasis.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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